Video-rate Scanning Confocal Microscopy and Microendoscopy
نویسندگان
چکیده
منابع مشابه
Video-rate scanning confocal microscopy and microendoscopy.
Confocal microscopy has become an invaluable tool in biology and the biomedical sciences, enabling rapid, high-sensitivity, and high-resolution optical sectioning of complex systems. Confocal microscopy is routinely used, for example, to study specific cellular targets, monitor dynamics in living cells, and visualize the three dimensional evolution of entire organisms. Extensions of confocal im...
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Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed specimens ranging in thickness up to 100 micrometers. Modern instruments are equipped with 3-5 laser systems controlled by high-speed acousto-optic tunable filters (AOTFs), which allow very precise regula...
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Many technological advancements of the past decade have contributed to improvements in the photon efficiency of the confocal laser scanning microscope (CLSM). The resolution of images from the new generation of CLSMs is approaching that achieved by the microscope itself because of continued development in digital imaging methods, laser technology and the availability of brighter and more photos...
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Taking advantage of the laser scanning methodology, confocal microscopy has been widely applied in cutting-edge biological research for its three-dimensional, high-resolution imaging capability (Pawley 1995). The principles of confocal were put forward by Marvin Minsky in 1955 (Minsky 1988), yet the experimental demonstration was not accomplished until 20 years later by Cremer brother (Cremer, ...
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Introduction The visualisation of sample elements using various forms of microscopy relies not only on magnification but also contrast. As such, differing forms of microscopy offer different information about a sample. Laser scanning confocal microscopy (LSCM) provides information about the 3D location of a particular, labelled component within a sample and has the additional advantage of exclu...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2011
ISSN: 1940-087X
DOI: 10.3791/3252